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Confocal Microscope

Within our bio-optics suite we have an inverted Nikon A1R confocal microscope, suitable for live-cell imaging.

The microscope has brightfield transmission mode, phase contrast, DIC contrast and epifluorescence. In confocal mode, which allows clear deep tissue imaging, we measure down to around 200 nm in the lateral dimension and 1 – 2 microns in depth

Features:

  • Brightfield and epifluorescence mode with BSI Prime 22mm CMOS camera
  • Live cell imaging
  • 4 laser excitation sources
  • Resonant and Galvano scanning modes plus spectral imaging
  • Multi-Photon ready

In addition, the output from the microscope can be routed to Picoquant photon counting detectors. This allows us to perform time-resolved microscopy techniques such as Fluorescence Lifetime Imaging (FLIM) and Fluorescence Correlation Spectroscopy (FCS). The out can be directly taken to the Picoquant spectrometer to allow true spectral imaging.