Cell free expression
Developing a cell free expression system
A novel approach for the production of recombinant proteins
An IUK funded collaborative project brought CPI, Ipsen Biopharm and Touchlight Genetics together to examine the application of cell free expression (CFE) to produce a highly potent product from doggybone DNA (dbDNA™), a novel linear DNA vector.
Cell free expression (CFE) has the potential to revolutionise the supply chain for specific protein products. As stratified and personalised medicines become a reality, the necessity for small batch sizes makes traditional manufacturing approaches prohibitively expensive; the use of CFE offers a cost-effective solution to this manufacturing challenge.
This project focused on exploring the potential to exploit CFE to manufacture commercial products. The technique has been used as a research tool for many years but had limited interest as a commercial manufacturing platform. A fully scalable CFE platform was therefore developed to facilitate rapid and flexible manufacture of the next generation of biologics.
![](https://optimise2.assets-servd.host/smart-hoopoe/production/content/images/biologics-lab-11.jpg?w=1920&h=1080&q=60&fm=jpg&fit=crop&dm=1559218080&s=ecce9baa9bf6a6c075abefe60150cb54 1920w, https://optimise2.assets-servd.host/smart-hoopoe/production/content/images/biologics-lab-11.jpg?w=1120&h=630&q=60&fm=jpg&fit=crop&dm=1559218080&s=4c1c8a666f8637babe1a0f8cfaced686 1120w, https://optimise2.assets-servd.host/smart-hoopoe/production/content/images/biologics-lab-11.jpg?w=800&h=450&q=60&fm=jpg&fit=crop&dm=1559218080&s=9eb4c9342fb22696bfa3d06092caf297 800w, https://optimise2.assets-servd.host/smart-hoopoe/production/content/images/biologics-lab-11.jpg?w=1600&h=900&q=45&fm=jpg&fit=crop&dm=1559218080&s=8253af9e29420e2f2bb7f5a666498224 1600w, https://optimise2.assets-servd.host/smart-hoopoe/production/content/images/biologics-lab-11.jpg?w=480&h=270&q=60&fm=jpg&fit=crop&dm=1559218080&s=a2c98c8207c852547f74367af9d92053 480w, https://optimise2.assets-servd.host/smart-hoopoe/production/content/images/biologics-lab-11.jpg?w=960&h=540&q=45&fm=jpg&fit=crop&dm=1559218080&s=5f2162095ac8b2b2e688555928fc3f09 960w)
“ As well as developing a new manufacturing route for Ipsen’s biopharmaceuticals, the Ipsen team have benefitted significantly though the collaborative nature of this research project. Technical discussions and site visits have introduced new equipment and broadened the experience of the scientists working on this project. ”
![David Gruber David Gruber](https://optimise2.assets-servd.host/smart-hoopoe/production/content/images/david-gruber.jpeg?w=100&h=100&q=60&fm=jpg&fit=crop&dm=1632737119&s=63eba53a9597e2ff4c0bb01455ac3111)
Partners
![Touchlight Genetics](https://optimise2.assets-servd.host/smart-hoopoe/production/content/logos/touchlight-genetics-logo.jpg?w=120&h=40&q=100&fm=png&fit=crop&dm=1578312522&s=4f478bc060987d4efc0dddf1eed6b6ca)
![Ipsen](https://optimise2.assets-servd.host/smart-hoopoe/production/content/logos/ipsen-logo.jpg?w=400&h=118&q=100&fm=png&fit=crop&dm=1578307471&s=735456cd91fe6a1eb537e3583eb7e8a1)
Achievements
Inputs
- Access to CPI’s state of the art bioprocessing facilities
- Shared expert technical and industry knowledge
- Project scoping, consortia building and proposal development
- Current dbDNA™ production process
- Provision of a test candidate for assessment of CFE
Outputs
- Generation of a robust and scalable lysate manufacturing process
- Establishment of high throughput screening tools for the rapid assessment of optimal reaction conditions
- Development of an optimised and scalable protocol for CFE
- Expertise and understanding of the challenges associated with cell free scale-up
- Technical knowledge in CFE systems and their optimisation in gene expression
- Improved closed loop dbDNA™ vector production process
Outcomes
- A new manufacturing route for biopharmaceuticals
- Benefits to the supply chain as biopharmaceuticals and vaccines can be manufactured faster with increased flexibility
- Benefits to patients as the supply of biological medicines can be improved
![](https://optimise2.assets-servd.host/smart-hoopoe/production/content/images/cpi-lab-med.jpg?w=1920&h=1080&q=60&fm=jpg&fit=crop&dm=1559223025&s=0ebe2f402630080be642fa705ccbd6de 1920w, https://optimise2.assets-servd.host/smart-hoopoe/production/content/images/cpi-lab-med.jpg?w=1120&h=630&q=60&fm=jpg&fit=crop&dm=1559223025&s=ccbf933216c12118b92e2beb5dab785f 1120w, https://optimise2.assets-servd.host/smart-hoopoe/production/content/images/cpi-lab-med.jpg?w=800&h=450&q=60&fm=jpg&fit=crop&dm=1559223025&s=8a26796249d0ec1914c0b57a5dbc86df 800w, https://optimise2.assets-servd.host/smart-hoopoe/production/content/images/cpi-lab-med.jpg?w=1600&h=900&q=45&fm=jpg&fit=crop&dm=1559223025&s=0fea5ff3827b7050f4d237f833d7cf4d 1600w, https://optimise2.assets-servd.host/smart-hoopoe/production/content/images/cpi-lab-med.jpg?w=480&h=270&q=60&fm=jpg&fit=crop&dm=1559223025&s=48e62a21a516e7d7c27d1f6560e3d25e 480w, https://optimise2.assets-servd.host/smart-hoopoe/production/content/images/cpi-lab-med.jpg?w=960&h=540&q=45&fm=jpg&fit=crop&dm=1559223025&s=3ec16f003ecfddd17d170ef9ff3798e8 960w)
The challenge
Current processes rely on protein expression in cells either endogenously encoded or genetically modified and manufacturing processes for biopharmaceutical products such as therapeutic neurotoxins present significant manufacturing challenges. A CFE platform has the potential to be both scalable and flexible towards the application of niche products and could prove a very powerful tool for industry. CPI, Ipsen and Touchlight worked together to address these challenges with the aim of assessing the viability of using CFE for commercial manufacture of the test candidate product.
How CPI helped
CPI utilised their state-of-the-art facilities and expertise to support the development of a CFE platform which can be applied across a range of products and scales. The scope included generating a simplified scalable production method, from laboratory to pilot scale suitable for the manufacture of a range of recombinant proteins. CPI utilised its high throughput screening tools combining liquid handling platforms and statistical experimental design for optimising reaction conditions for CFE which resulted in scale-up and transfer of a robust CFE system for production of a highly potent product.
Through this collaborative project, a new manufacturing route for biopharmaceuticals was developed which significantly benefits the supply chain as biopharmaceuticals and vaccines can be manufactured faster with increased flexibility.
Related
Technologies
Markets
![](https://optimise2.assets-servd.host/smart-hoopoe/production/content/images/pharma-processing-02.jpg?w=1920&h=1080&q=60&fm=jpg&fit=crop&dm=1574776918&s=50954d9bac99faa5dca7ed1ea0a5d4d2 1920w, https://optimise2.assets-servd.host/smart-hoopoe/production/content/images/pharma-processing-02.jpg?w=1120&h=630&q=60&fm=jpg&fit=crop&dm=1574776918&s=4322a2918b760f796567bc4f946b0568 1120w, https://optimise2.assets-servd.host/smart-hoopoe/production/content/images/pharma-processing-02.jpg?w=800&h=450&q=60&fm=jpg&fit=crop&dm=1574776918&s=ce91d066b12497fbc54a0de59b6e45fa 800w, https://optimise2.assets-servd.host/smart-hoopoe/production/content/images/pharma-processing-02.jpg?w=1600&h=900&q=45&fm=jpg&fit=crop&dm=1574776918&s=1e774c08ae4b6417ddf4deccd2d952e4 1600w, https://optimise2.assets-servd.host/smart-hoopoe/production/content/images/pharma-processing-02.jpg?w=480&h=270&q=60&fm=jpg&fit=crop&dm=1574776918&s=9117fc65a9e6fb0021f9790a8fc320e9 480w, https://optimise2.assets-servd.host/smart-hoopoe/production/content/images/pharma-processing-02.jpg?w=960&h=540&q=45&fm=jpg&fit=crop&dm=1574776918&s=0a1064d13fa742a61ad1dbe0444b3d23 960w)